Biosecurity is a strategic and integrated approach to analysing and managing relevant risks to human, animal and plant life and health and associated risks to the environment. This toolkit has been developed by FAO to support countries to develop and implement national biosecurity frameworks in accordance with their international obligations and particular needs. It presents the benefits of a harmonised and integrated approach to biosecurity and illustrates the experiences of countries that have adopted such an approach in recent times. The toolkit comprises three separate but related parts. The first part, Biosecurity principles and components, is an introductory text providing a contemporary context for the development and implementation of a harmonized and integrated biosecurity approach across all sectors. The second part is a Guide to assess biosecurity capacity, which offers a process for assessing dimensions of biosecurity capacity across all sectors and sector organizations. The third part, an overview and framework manual for biosecurity risk analysis, presents a generic framework to structure and guide the application of risk analysis principles in biosecurity.